Research Fellow

Contact Details

Office 211, Level 2 John Hines Building (62)
P +61 07 3365 4436
F +61 07 3365 3556

Short Biography

Arief completed his Master of Agricultural Science in 1999 at the University of Queensland. He finished his PhD studies from the same university in 2007. During his PhD he studied Nod factor perception in soybean in which he produced one international patent for his discovery on Nod factor receptor of soybean in relation to acid tolerant symbiosis. In February 2009, Arief accepted a position of Postdoctoral Research Fellow at the ARC Centre of Excellence for Integrative Legume Research where he's currently working on the analysis of gene function in early nodulation and stress tolerant of symbiotic nitrogen fixation.

Research Skills

Gene cloning and functional characterisation, DNA fingerprinting, hairy root transformation, nitrogen fixation measurement, bacteria strain identification, analysis of stress environment related soils, and plant growth analysis.

Current Projects

Soybean Nodulation Control

Bradyrhizobium japonicum Nod Factor responses
   a)   Analysis of soybean Nod Factor (NF) receptors GmNFR1 and GnNFR5
   b)   Analysis of soil stress responses specifically acid soil
   c)   Analysis of the role of GmNORK genes
   d)   Analysis of gene function in early nodulation

Key Publications

1. Indrasumunar A, Searle I, Lin MH, Kereszt A, Men A, Carroll BJ, Gresshoff PM (2011) Nodulation Factor Receptor Kinase 1? Controls Nodule Organ Number in Soybean (Glycine max L. Merr.). Plant Journal, 65: 39-50.
2. Indrasumunar A, Kereszt A, Miyagi M, Nguyen CDT, Searle I, Men A, Carroll BJ, and Gresshoff PM (2010) Inactivation of duplicated Nod-Factor Receptor 5 (NFR5) genes in recessive loss-of-function non-nodulation mutants of allotetraploid soybean (Glycine max L. Merr.). Plant and Cell Physiology 51(2): 201-214.
3. Indrasumunar A, Menzies N, Dart PJ (2012) Laboratory prescreening of Bradyrhizobium japonicum for low pH, Al and Mn tolerance can be used to predict their survival in acid soils. Soil Biology and Biochemistry, 48: 135-141.
4. Indrasumunar A, Menzies N, Dart PJ (2012) Calcium affects the competitiveness of acid-sensitive and acid-tolerant strains of Bradyrhizobium japonicum in nodulating and fixing nitrogen with two soybean cultivars in acid soil. Soil Biology and Biochemistry, 46: 115-122.
5. Indrasumunar A, Dart PJ, Menzies N (2011) Symbiotic effectiveness of Bradyrhizobium japonicum in acid soils can be predicted from their sensitivity to acid soil stress factors in acidic agar media. Soil Biology and Biochemistry, 43: 2046-2052.
6. Lee WK, Jeong N, Indrasumunar A, Gresshoff PM, Jeong SC (2011) Glycine max non-nodulation locus rj1: a recombinogenic region encompassing a SNP in a lysine motif receptor-like kinase (GmNFR1?). Theoretical and Appled Genetics, 122: 875-884.
7. Indrasumunar A, Gresshoff PM (2011) Evolutionary duplication of lipo-oligochitin-like receptor genes in soybean differentiates their function in cell division and cell invasion. Plant Signaling and Behavior, 6, 534-537.
8. Lin M-H, Gresshoff PM, Indrasumunar A, Ferguson BJ (2011) pHairyRed: a novel binary vector containing the DsRed2 reporter genefor visual selection of transgenic hairy roots. Molecular Plant, 4 (3): 537-545.
9. Kereszt A, Dongxue L, Indrasumunar A, Nguyen CDT, Nontachaiyapoom S, Kinkema M, Gresshoff PM (2007) Agrobacterium rhizogenes-mediated transformation of soybean to study root biology. Nature Protocols 2: 948-952.
10. Ferguson, BJ, Indrasumunar A, Hayashi S, Lin Y-H, Lin M-H, Reid D and Gresshoff PM (2010) Molecular analysis of legume nodule development and autoregulation. Journal of Integrative Plant Biology 52 (1): 61-76.
11. Indrasumunar A, Gresshoff PM (2010) Duplicated Nod-Factor Receptor 5 (NFR5) genes are mutated in soybean (Glycine max L. Merr.). Plant Signalling and Behavior 5 (5): 535-536.
12. Gresshoff PM, Ferguson BJ, Indrasumunar A and Jiang Q (2009) Modern genetics and biotechnology of soybean nitrogen fixation and nodulation. Chinese Journal of Nature 31(6): 320-326.


13. Indrasumunar A, Kereszt A, Gresshoff PM (2007) Soybean Nodulation Factor Receptor Proteins, Encoding Nucleic Acids and Uses Therefore. Patent Cooperation Treaty. International application number: PCT/AU2006/001963, International filing date: 22.12.2006, Publication number: (WO/2007/070960), Publication Date: 28.06.2007.

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