Mr Salman Alamery
2011 commencement of PhD candidature. The University of Queensland
2010 Master of Science with Honours, Biotechnology, Griffith University.
The major aim of my project is to identify and sequence resistance genes in Brassica napus against Leptosphaeria maculans, a pathogen causing blackleg disease. This is the most serious fungal disease of canola and causes significant economic loss. My project focuses on the qualitative, single-gene, race specific resistance, where the result of infection (resistance or susceptibility) of the plant by pathogen is dependent on the presence of a specific resistance (R) gene in the plant and a corresponding avirulence (Avr) gene in the pathogen.
In this project sequence based markers, underlying blackleg resistance QTL in canola mapping populations segregating for Rlm2, 4, 7 and 9 resistance will be used to identify the physical region on B. rapa chromosomes A7 and A10 containing the resistance genes. This will be performed through aligning the primer sequences of the markers to the B. rapa genome sequence and identifying contigs which contain the markers in the approximate correct order. Genes in the QTL regions will be identified and annotated to determine the most likely candiates for blackleg resistance based on literature searches of known resistance genes. PCR primers will be designed for amplification and sequence verification of the best candidate TIR-NBS-LRR genes in B. napus, known to be resistant to blackleg and predicted to contain Rlm2, 4, 7 and 9, as well as susceptible lines. In addition next generation sequence data of resistant and susceptible lines will be mapped to the QTL region. SNPs from the next generation sequence data and PCR amplification of the genes will be identified and associated with resistance/susceptibility for validation of gene candidates. Genetic transformation and gene expression will be used to validate that the resistance is conferred by the identified candidate resistance gene.
Dr. Jacqueline Batley
Prof. David Edwards